PAX7 Antikörper (AA 300-600) (CF®740)

Produktnummer ABIN7658345

Der Maus Monoklonal Anti-PAX7 Antikörper wurde für WB validiert. Er ist geeignet, PAX7 in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio) und Huhn zu detektieren.

Kurzübersicht für PAX7 Antikörper (AA 300-600) (CF®740) (ABIN7658345)

Target: PAX7 (Paired Box 7 (PAX7))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX7 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: PAX7-497

Produktdetails anti-PAX7 Antikörper (CF®740)

Bindungsspezifität: AA 300-600

Verwendungszweck: PAX7(PAX7/497), CF740 conjugate

Produktmerkmale: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Rhabdomyosarcoma (RMS)|U2OS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Andere Bezeichnung: PAX7

Hintergrund:

Synonyms: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

Gene Symbol: PAX7

Molekulargewicht: 57 kDa

Gen-ID: 5081

UniProt: P23759